Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1939609..1940227 Replicon chromosome
Accession NZ_CP043406
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FZN32_RS09715 Protein ID WP_001291435.1
Coordinates 1940009..1940227 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FZN32_RS09710 Protein ID WP_000344800.1
Coordinates 1939609..1939983 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS09700 1934698..1935891 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FZN32_RS09705 1935914..1939063 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
FZN32_RS09710 1939609..1939983 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FZN32_RS09715 1940009..1940227 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FZN32_RS09720 1940399..1940950 + 552 WP_000102559.1 maltose O-acetyltransferase -
FZN32_RS09725 1941066..1941536 + 471 WP_000136192.1 YlaC family protein -
FZN32_RS09730 1941700..1943250 + 1551 Protein_1905 cyclic-guanylate-specific phosphodiesterase PdeB -
FZN32_RS09735 1943292..1943645 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FZN32_RS09745 1944024..1944335 + 312 WP_000409911.1 MGMT family protein -
FZN32_RS09750 1944366..1944938 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138684 WP_001291435.1 NZ_CP043406:1940009-1940227 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138684 NZ_CP058209:c852134-852031 [Escherichia marmotae]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138684 WP_000344800.1 NZ_CP043406:1939609-1939983 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138684 NZ_CP043406:1939609-1939983 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References