Detailed information of TA system TA138681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 794473..795111
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: FZN32_RS03945
• Protein ID: WP_000813797.1
• Coordinates: 794935..795111 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FZN32_RS03940
• Protein ID: WP_076611057.1
• Coordinates: 794473..794889 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS03920	789625..790566	-	942	WP_001251335.1	ABC transporter permease	-
FZN32_RS03925	790567..791580	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
FZN32_RS03930	791598..792743	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
FZN32_RS03935	792988..794394	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
FZN32_RS03940	794473..794889	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FZN32_RS03945	794935..795111	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FZN32_RS03950	795333..795563	+	231	WP_000494244.1	YncJ family protein	-
FZN32_RS03955	795655..797616	-	1962	WP_173002388.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FZN32_RS03960	797689..798225	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
FZN32_RS03965	798278..799489	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T138681 WP_000813797.1 NZ_CP043406:c795111-794935 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T138681 NZ_CP058209:479806-479982 [Escherichia marmotae]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT138681 WP_076611057.1 NZ_CP043406:c794889-794473 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT138681 NZ_CP043406:c794889-794473 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715