Detailed information of TA system TA138670

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 121571..122402
• Replicon: chromosome
• Accession: NZ_CP043406
• Organism: Escherichia coli strain NMBU-W13E19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: FZN32_RS00630
• Protein ID: WP_000854815.1
• Coordinates: 121571..121945 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A061Y7A8
• Locus tag: FZN32_RS00635
• Protein ID: WP_001280918.1
• Coordinates: 122034..122402 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZN32_RS00590	116967..118133	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
FZN32_RS00595	118252..118725	+	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
FZN32_RS00600	118923..119981	+	1059	WP_001200889.1	FUSC family protein	-
FZN32_RS00605	120153..120482	+	330	WP_000450409.1	DUF496 family protein	-
FZN32_RS00610	120583..120765	-	183	WP_001296208.1	hypothetical protein	-
FZN32_RS00615	120819..120965	+	147	Protein_122	transposase domain-containing protein	-
FZN32_RS00620	121254..121367	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FZN32_RS00625	121380..121574	-	195	WP_000988600.1	hypothetical protein	-
FZN32_RS00630	121571..121945	-	375	WP_000854815.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FZN32_RS00635	122034..122402	-	369	WP_001280918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FZN32_RS00640	122418..123062	-	645	WP_000086752.1	hypothetical protein	-
FZN32_RS00645	123081..123302	-	222	WP_000692345.1	DUF987 domain-containing protein	-
FZN32_RS00650	123365..123841	-	477	WP_001186200.1	RadC family protein	-
FZN32_RS00655	123857..124330	-	474	WP_001542276.1	antirestriction protein	-
FZN32_RS00660	124424..124669	-	246	WP_001164966.1	hypothetical protein	-
FZN32_RS00665	124669..125487	-	819	WP_001542275.1	DUF945 domain-containing protein	-
FZN32_RS00670	125708..126118	-	411	WP_000846703.1	hypothetical protein	-
FZN32_RS00675	126134..126484	-	351	Protein_134	hypothetical protein	-
FZN32_RS00680	126567..127313	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T138670 WP_000854815.1 NZ_CP043406:c121945-121571 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T138670 NZ_CP058207:c689333-689124 [Escherichia marmotae]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13811.70 Da
• Isoelectric Point: 6.4767

>AT138670 WP_001280918.1 NZ_CP043406:c122402-122034 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT138670 NZ_CP058207:689325-689382 [Escherichia marmotae]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: A0A066T988

Antitoxin

• AlphaFold DB: A0A061Y7A8