Detailed information of TA system TA138648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2388397..2388971
• Replicon: chromosome
• Accession: NZ_CP043398
• Organism: Leclercia adecarboxylata strain G426

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FY047_RS11160
• Protein ID: WP_103792339.1
• Coordinates: 2388645..2388971 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6G7TED5
• Locus tag: FY047_RS11155
• Protein ID: WP_059307895.1
• Coordinates: 2388397..2388645 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY047_RS11140	2385301..2386845	+	1545	WP_059307898.1	nitrate reductase subunit beta	-
FY047_RS11145	2386845..2387540	+	696	WP_059307897.1	nitrate reductase molybdenum cofactor assembly chaperone	-
FY047_RS11150	2387537..2388217	+	681	WP_032618185.1	respiratory nitrate reductase subunit gamma	-
FY047_RS11155	2388397..2388645	+	249	WP_059307895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FY047_RS11160	2388645..2388971	+	327	WP_103792339.1	endoribonuclease MazF	Toxin
FY047_RS11165	2389060..2389509	-	450	WP_032618182.1	hypothetical protein	-
FY047_RS11170	2389743..2390588	-	846	WP_103792338.1	N-hydroxyarylamine O-acetyltransferase	-
FY047_RS11175	2390785..2392440	-	1656	WP_103792337.1	iron ABC transporter permease	-
FY047_RS11180	2392479..2393447	-	969	WP_032618179.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11861.62 Da
• Isoelectric Point: 7.8831

>T138648 WP_103792339.1 NZ_CP043398:2388645-2388971 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVVSPATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T138648 NZ_CP058188:c168951-168466 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATAGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9137.39 Da
• Isoelectric Point: 4.4777

>AT138648 WP_059307895.1 NZ_CP043398:2388397-2388645 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT138648 NZ_CP043398:2388397-2388645 [Leclercia adecarboxylata]
ATGCCGCAAGTCACCGTCAAAAAATGGGGTAACAGCCCTTCTGTCAGGCTGCCTGTCGCAGTTATGCGCGAGGCTGAATT
GCACGTTGACGATGTGGTGAACGTTAACGTAGACGAAGAAGGACGCATTATTTTGACCCCCGTCAGGAAGGACGAACCGA
CGCTGGAATCACTGCTCGCTGCGATCACCGACGACAATCTGCATCACGAAGTCACCTTTGGAGAACCGCAAGGTAAGGAG
CTGTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G7TED5