Detailed information of TA system TA138634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2429917..2430491
• Replicon: chromosome
• Accession: NZ_CP043397
• Organism: Leclercia adecarboxylata strain L21

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FY044_RS11680
• Protein ID: WP_059307894.1
• Coordinates: 2430165..2430491 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FY044_RS11675
• Protein ID: WP_164540382.1
• Coordinates: 2429917..2430165 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY044_RS11660	2426820..2428364	+	1545	WP_059307898.1	nitrate reductase subunit beta	-
FY044_RS11665	2428364..2429059	+	696	WP_059307897.1	nitrate reductase molybdenum cofactor assembly chaperone	-
FY044_RS11670	2429056..2429736	+	681	WP_164540380.1	respiratory nitrate reductase subunit gamma	-
FY044_RS11675	2429917..2430165	+	249	WP_164540382.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FY044_RS11680	2430165..2430491	+	327	WP_059307894.1	endoribonuclease MazF	Toxin
FY044_RS11685	2430581..2431030	-	450	WP_164540384.1	hypothetical protein	-
FY044_RS11690	2431264..2432109	-	846	WP_059307893.1	N-hydroxyarylamine O-acetyltransferase	-
FY044_RS11695	2432306..2433961	-	1656	WP_164540386.1	iron ABC transporter permease	-
FY044_RS11700	2434000..2434968	-	969	WP_032618179.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• Antitoxin PemI-like (4-81)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11875.64 Da
• Isoelectric Point: 7.8831

>T138634 WP_059307894.1 NZ_CP043397:2430165-2430491 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVISPATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T138634 NZ_CP058180:4552793-4553011 [Klebsiella grimontii]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9151.42 Da
• Isoelectric Point: 4.4777

>AT138634 WP_164540382.1 NZ_CP043397:2429917-2430165 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVLNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT138634 NZ_CP058180:4552392-4552766 [Klebsiella grimontii]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG