Detailed information of TA system TA138630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 755987..756630
• Replicon: chromosome
• Accession: NZ_CP043397
• Organism: Leclercia adecarboxylata strain L21

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FY044_RS03565
• Protein ID: WP_015387203.1
• Coordinates: 755987..756403 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3E1ZVJ5
• Locus tag: FY044_RS03570
• Protein ID: WP_032616516.1
• Coordinates: 756400..756630 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY044_RS03545	752010..752600	-	591	WP_059308736.1	TetR/AcrR family transcriptional regulator	-
FY044_RS03550	752725..753606	-	882	WP_071886490.1	NmrA family NAD(P)-binding protein	-
FY044_RS03555	753844..754749	-	906	WP_059308734.1	SDR family oxidoreductase	-
FY044_RS03560	754892..755788	+	897	WP_164539869.1	AraC family transcriptional regulator	-
FY044_RS03565	755987..756403	-	417	WP_015387203.1	type II toxin-antitoxin system VapC family toxin	Toxin
FY044_RS03570	756400..756630	-	231	WP_032616516.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FY044_RS03575	757083..757235	+	153	WP_059308732.1	Hok/Gef family protein	-
FY044_RS03580	757327..757932	-	606	WP_032616518.1	3-isopropylmalate dehydratase small subunit	-
FY044_RS03585	757943..759343	-	1401	WP_032616519.1	3-isopropylmalate dehydratase large subunit	-
FY044_RS03590	759346..760437	-	1092	WP_059308730.1	3-isopropylmalate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	737669..757235	19566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14924.31 Da
• Isoelectric Point: 7.2449

>T138630 WP_015387203.1 NZ_CP043397:c756403-755987 [Leclercia adecarboxylata]
VIKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGLLVEAFCARLDAILAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWAN

Nucleotide

• Length: 417 bp

>T138630 NZ_CP043397:c756403-755987 [Leclercia adecarboxylata]
GTGATCAAGACCTATATGCTGGACACCAACATTTGCTCGTTCATCATGCGTGAGCAGCCGGAAGCTGTCATTAAACGGCT
GGAGCAGGCAGTGCTGCGGAATCACCGCATCGTGGTTTCGGCCATTACTTATGCAGAGATGCGTTTCGGTGCGATCGGAA
AGAAGGCATCTCCCCGCCACGGCCTGCTTGTCGAAGCTTTCTGCGCCCGCCTCGATGCAATCCTCGCCTGGGACCGCGCC
GCGGTGGACGCAACCACGGAGATTAAGGCCGCACTGACTGCGGCCGGGACGCCAATCGGTCCGAACGATACGGCGATCGC
CGGGCATGCCATCGCCGCCGGCGCAGTGCTGGTAACGAACAATGTACGTGAGTTTGAGCGTGTGCCTGGCCTTGTCCTGG
AAGACTGGGCTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8687.72 Da
• Isoelectric Point: 4.4563

>AT138630 WP_032616516.1 NZ_CP043397:c756630-756400 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFTQLEKADSDFMSERGDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT138630 NZ_CP043397:c756630-756400 [Leclercia adecarboxylata]
ATGAGAACCGTATCGATATTCAAAAACGGCAACAATCGCGCTATCCGTCTGCCCCGCGACCTGGATTTTGAGGGAGTGAG
CGAACTGGAAATCGTCCGGGAAGGTGACAGCATCATCCTTCGTCCCGTTCGTCCAACATGGGGTTCGTTTACTCAGCTCG
AAAAAGCCGACTCAGACTTTATGTCCGAACGTGGGGACGTCGTCAGCGATGAAGGACGGTTTGACTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3E1ZVJ5