Detailed information of TA system TA138628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4886386..4886994
• Replicon: chromosome
• Accession: NZ_CP043396
• Organism: Pseudomonas monteilii strain 170620603RE

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FY043_RS23250
• Protein ID: WP_164525070.1
• Coordinates: 4886704..4886994 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A6G6V4Q8
• Locus tag: FY043_RS23245
• Protein ID: WP_060499944.1
• Coordinates: 4886386..4886694 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY043_RS23195	4882259..4882651	+	393	WP_164525064.1	helix-turn-helix transcriptional regulator	-
FY043_RS23200	4882689..4882949	+	261	WP_164525065.1	hypothetical protein	-
FY043_RS23205	4883056..4883850	+	795	WP_016715556.1	Bro-N domain-containing protein	-
FY043_RS23210	4883931..4884110	+	180	WP_016715555.1	carbon storage regulator	-
FY043_RS23215	4884100..4884321	+	222	WP_164525066.1	Arc family DNA-binding protein	-
FY043_RS23220	4884665..4884871	+	207	Protein_4535	carbon storage regulator	-
FY043_RS23225	4884868..4885050	+	183	WP_164525067.1	hypothetical protein	-
FY043_RS23230	4885043..4885606	+	564	WP_164525068.1	HNH endonuclease	-
FY043_RS23235	4885596..4885838	+	243	WP_019752348.1	hypothetical protein	-
FY043_RS23240	4885913..4886125	+	213	WP_164525069.1	AlpA family phage regulatory protein	-
FY043_RS23245	4886386..4886694	-	309	WP_060499944.1	putative addiction module antidote protein	Antitoxin
FY043_RS23250	4886704..4886994	-	291	WP_164525070.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY043_RS23255	4887106..4889628	-	2523	WP_060499945.1	TonB-dependent receptor	-
FY043_RS23260	4889704..4890645	-	942	WP_164525071.1	FecR domain-containing protein	-
FY043_RS23265	4890645..4891157	-	513	WP_164525274.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4846218..4894431	48213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10711.19 Da
• Isoelectric Point: 8.9632

>T138628 WP_164525070.1 NZ_CP043396:c4886994-4886704 [Pseudomonas monteilii]
VKTILTTDDFDAWFSKLSDKRSAMRIQARIDRAEAGNFGDCQAVGEGVSEMRIHYGPGYRVYFTQRGLEIVILLAGGDKS
TQSKDIKTALDIARRL

Nucleotide

• Length: 291 bp

>T138628 NZ_CP058175:c4733126-4732755 [Enterobacter hormaechei]
ATGGAGCACATGGCTGTGTTTGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTCATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAAAACGGCAGCCGTGATGGAGGCCTTTGAAATTATTGATATAACAAGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10817.32 Da
• Isoelectric Point: 6.2202

>AT138628 WP_060499944.1 NZ_CP043396:c4886694-4886386 [Pseudomonas monteilii]
MGNVKLKKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVTRALGLQLHADVASTHTG

Nucleotide

• Length: 309 bp

>AT138628 NZ_CP043396:c4886694-4886386 [Pseudomonas monteilii]
ATGGGTAACGTCAAGTTGAAAAAATGGGACAGTGCCGAACACCTCAAGACGGACGAGGACATGGCGCTTTACCTCGAAGC
TTGCATTGAAGAGGCGGGTGATGATGCGGCTTTTATCGCCAAGGCGCTCGGCACCATTGCGCGTGCAAAAGGCATGGCTC
AGTTGGCCCGTGATACCGGGCTTGGGCGTGAAAGCCTGTACAAGGCGTTGTCCGGCGAGGGGAACCCAAGCTTCGCCACC
ATCCTGAAGGTGACCAGAGCGCTTGGCCTACAGCTTCATGCTGACGTTGCATCCACTCATACTGGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A6G6V4Q8