Detailed information of TA system TA138627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3490542..3491176
• Replicon: chromosome
• Accession: NZ_CP043396
• Organism: Pseudomonas monteilii strain 170620603RE

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY043_RS16390
• Protein ID: WP_003291451.1
• Coordinates: 3490542..3490946 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: FY043_RS16395
• Protein ID: WP_003285520.1
• Coordinates: 3490946..3491176 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY043_RS16365	3486802..3487407	-	606	WP_164524682.1	hypothetical protein	-
FY043_RS16370	3487445..3487936	-	492	WP_164524683.1	hypothetical protein	-
FY043_RS27445	3488663..3488908	-	246	WP_206520096.1	hypothetical protein	-
FY043_RS16380	3489098..3489430	+	333	WP_036992178.1	hypothetical protein	-
FY043_RS16385	3489490..3490458	+	969	WP_164524684.1	DUF932 domain-containing protein	-
FY043_RS16390	3490542..3490946	-	405	WP_003291451.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FY043_RS16395	3490946..3491176	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FY043_RS16400	3491333..3492337	+	1005	WP_003291452.1	YqaJ viral recombinase family protein	-
FY043_RS16405	3492429..3493334	+	906	WP_164524685.1	hydrolase or metal-binding protein	-
FY043_RS16410	3493345..3493842	+	498	WP_116801388.1	DNA repair protein RadC	-
FY043_RS16415	3493853..3494023	+	171	WP_164524686.1	hypothetical protein	-
FY043_RS16420	3494119..3495126	-	1008	WP_164525254.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aph(6)-Id / aph(3'')-Ib / sul1 / cmlA1 / aac(6')-IIa	-	3474232..3535882	61650
	-	flank	IS/Tn	-	-	3495509..3496369	860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14968.21 Da
• Isoelectric Point: 7.2774

>T138627 WP_003291451.1 NZ_CP043396:c3490946-3490542 [Pseudomonas monteilii]
MLKYMLDTNMCIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVSA

Nucleotide

• Length: 405 bp

>T138627 NZ_CP058175:c3856664-3856254 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAGATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT138627 WP_003285520.1 NZ_CP043396:c3491176-3490946 [Pseudomonas monteilii]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT138627 NZ_CP058175:c3856911-3856645 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: L1LS63