Detailed information of TA system TA138623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4887495..4888103
• Replicon: chromosome
• Accession: NZ_CP043395
• Organism: Pseudomonas monteilii strain 170918607

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FY041_RS23265
• Protein ID: WP_164525070.1
• Coordinates: 4887813..4888103 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A6G6V4Q8
• Locus tag: FY041_RS23260
• Protein ID: WP_060499944.1
• Coordinates: 4887495..4887803 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY041_RS23210	4883368..4883760	+	393	WP_164525064.1	helix-turn-helix transcriptional regulator	-
FY041_RS23215	4883798..4884058	+	261	WP_164525065.1	hypothetical protein	-
FY041_RS23220	4884165..4884959	+	795	WP_016715556.1	Bro-N domain-containing protein	-
FY041_RS23225	4885040..4885219	+	180	WP_016715555.1	carbon storage regulator	-
FY041_RS23230	4885209..4885430	+	222	WP_164525066.1	Arc family DNA-binding protein	-
FY041_RS23235	4885774..4885980	+	207	Protein_4536	carbon storage regulator	-
FY041_RS23240	4885977..4886159	+	183	WP_164525067.1	hypothetical protein	-
FY041_RS23245	4886152..4886715	+	564	WP_164525068.1	HNH endonuclease	-
FY041_RS23250	4886705..4886947	+	243	WP_019752348.1	hypothetical protein	-
FY041_RS23255	4887022..4887234	+	213	WP_164525069.1	AlpA family phage regulatory protein	-
FY041_RS23260	4887495..4887803	-	309	WP_060499944.1	putative addiction module antidote protein	Antitoxin
FY041_RS23265	4887813..4888103	-	291	WP_164525070.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY041_RS23270	4888215..4890737	-	2523	WP_060499945.1	TonB-dependent siderophore receptor	-
FY041_RS23275	4890813..4891754	-	942	WP_164525071.1	FecR domain-containing protein	-
FY041_RS23280	4891754..4892266	-	513	WP_164525274.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4847327..4888103	40776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10711.19 Da
• Isoelectric Point: 8.9632

>T138623 WP_164525070.1 NZ_CP043395:c4888103-4887813 [Pseudomonas monteilii]
VKTILTTDDFDAWFSKLSDKRSAMRIQARIDRAEAGNFGDCQAVGEGVSEMRIHYGPGYRVYFTQRGLEIVILLAGGDKS
TQSKDIKTALDIARRL

Nucleotide

• Length: 291 bp

>T138623 NZ_CP058175:c93967-93647 [Enterobacter hormaechei]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGATACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCTTGGATAACATCTTTGGATGTTCATCGGGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10817.32 Da
• Isoelectric Point: 6.2202

>AT138623 WP_060499944.1 NZ_CP043395:c4887803-4887495 [Pseudomonas monteilii]
MGNVKLKKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVTRALGLQLHADVASTHTG

Nucleotide

• Length: 309 bp

>AT138623 NZ_CP058175:c94366-94001 [Enterobacter hormaechei]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGCATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTTGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
CTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
TTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G6V4Q8