Detailed information of TA system TA138622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3491651..3492285
• Replicon: chromosome
• Accession: NZ_CP043395
• Organism: Pseudomonas monteilii strain 170918607

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FY041_RS16405
• Protein ID: WP_003291451.1
• Coordinates: 3491651..3492055 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: FY041_RS16410
• Protein ID: WP_003285520.1
• Coordinates: 3492055..3492285 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY041_RS16380	3487911..3488516	-	606	WP_164524682.1	hypothetical protein	-
FY041_RS16385	3488554..3489045	-	492	WP_164524683.1	hypothetical protein	-
FY041_RS27455	3489772..3490017	-	246	WP_206520096.1	hypothetical protein	-
FY041_RS16395	3490207..3490539	+	333	WP_036992178.1	hypothetical protein	-
FY041_RS16400	3490599..3491567	+	969	WP_164524684.1	DUF932 domain-containing protein	-
FY041_RS16405	3491651..3492055	-	405	WP_003291451.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FY041_RS16410	3492055..3492285	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FY041_RS16415	3492442..3493446	+	1005	WP_003291452.1	YqaJ viral recombinase family protein	-
FY041_RS16420	3493538..3494443	+	906	WP_164524685.1	hydrolase or metal-binding protein	-
FY041_RS16425	3494454..3494951	+	498	WP_116801388.1	DNA repair protein RadC	-
FY041_RS16430	3494962..3495132	+	171	WP_164524686.1	hypothetical protein	-
FY041_RS16435	3495228..3496235	-	1008	WP_164525254.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aph(6)-Id / aph(3'')-Ib / sul1 / cmlA1 / aac(6')-IIa	-	3475341..3536991	61650
	-	flank	IS/Tn	-	-	3496618..3497478	860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14968.21 Da
• Isoelectric Point: 7.2774

>T138622 WP_003291451.1 NZ_CP043395:c3492055-3491651 [Pseudomonas monteilii]
MLKYMLDTNMCIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVSA

Nucleotide

• Length: 405 bp

>T138622 NZ_CP058175:30479-30793 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT138622 WP_003285520.1 NZ_CP043395:c3492285-3492055 [Pseudomonas monteilii]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT138622 NZ_CP043395:c3492285-3492055 [Pseudomonas monteilii]
ATGGAACAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGCGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGCAGATCAAGAGCGGGATGCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: L1LS63