Detailed information of TA system TA138612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2193984..2194513
• Replicon: chromosome
• Accession: NZ_CP043392
• Organism: Staphylococcus aureus strain NRS384-rpoB-H481N-NCV

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FZC50_RS11265
• Protein ID: WP_000621175.1
• Coordinates: 2193984..2194346 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FZC50_RS11270
• Protein ID: WP_000948331.1
• Coordinates: 2194343..2194513 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZC50_RS11245	2190962..2191732	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
FZC50_RS11250	2191707..2192186	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FZC50_RS11255	2192188..2192514	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FZC50_RS11260	2192633..2193634	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FZC50_RS11265	2193984..2194346	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FZC50_RS11270	2194343..2194513	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FZC50_RS11275	2194598..2195746	-	1149	WP_001281145.1	alanine racemase	-
FZC50_RS11280	2195812..2196171	-	360	WP_000581200.1	holo-ACP synthase	-
FZC50_RS11285	2196175..2196666	-	492	WP_001205910.1	PH domain-containing protein	-
FZC50_RS11290	2196653..2198236	-	1584	WP_001294626.1	PH domain-containing protein	-
FZC50_RS11295	2198229..2198708	-	480	WP_001287088.1	hypothetical protein	-
FZC50_RS11300	2198916..2199476	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T138612 WP_000621175.1 NZ_CP043392:c2194346-2193984 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T138612 NZ_CP058168:c53009-52539 [Enterobacter hormaechei]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCTTCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTATTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCTGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT138612 WP_000948331.1 NZ_CP043392:c2194513-2194343 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT138612 NZ_CP058168:c53449-53006 [Enterobacter hormaechei]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1