Detailed information of TA system TA138561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2732574..2733170
• Replicon: chromosome
• Accession: NZ_CP043382
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain WCHEX045001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: EKN98_RS13400
• Protein ID: WP_023315213.1
• Coordinates: 2732868..2733170 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EKN98_RS13395
• Protein ID: WP_032610152.1
• Coordinates: 2732574..2732861 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKN98_RS13390	2730946..2732577	+	1632	WP_032610151.1	Na/Pi cotransporter family protein	-
EKN98_RS13395	2732574..2732861	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
EKN98_RS13400	2732868..2733170	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKN98_RS13405	2733368..2734240	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
EKN98_RS13410	2734241..2734513	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
EKN98_RS13415	2734564..2735508	-	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
EKN98_RS13420	2735602..2736951	-	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T138561 WP_023315213.1 NZ_CP043382:c2733170-2732868 [Enterobacter hormaechei subsp. xiangfangensis]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T138561 NZ_CP043382:c2733170-2732868 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT138561 WP_032610152.1 NZ_CP043382:c2732861-2732574 [Enterobacter hormaechei subsp. xiangfangensis]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT138561 NZ_CP058153:c3754145-3753879 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A837F9W8