Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 127594..128333 Replicon plasmid pOXA1_095005
Accession NZ_CP043380
Organism Klebsiella pneumoniae strain WCHKP095005

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag B7D44_RS27500 Protein ID WP_013087172.1
Coordinates 127594..128079 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag B7D44_RS27505 Protein ID WP_012540086.1
Coordinates 128067..128333 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D44_RS27450 (B7D44_27725) 123195..123608 - 414 WP_004182074.1 helix-turn-helix domain-containing protein -
B7D44_RS27455 (B7D44_27730) 123609..123887 - 279 WP_004152721.1 helix-turn-helix transcriptional regulator -
B7D44_RS27460 (B7D44_27735) 123877..124197 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
B7D44_RS27465 (B7D44_27740) 124278..124502 - 225 WP_004152719.1 hypothetical protein -
B7D44_RS27470 (B7D44_27745) 124513..124725 - 213 WP_004152718.1 hypothetical protein -
B7D44_RS27475 (B7D44_27750) 124786..125142 - 357 WP_004152717.1 hypothetical protein -
B7D44_RS27480 (B7D44_27760) 125778..126128 - 351 WP_023320095.1 hypothetical protein -
B7D44_RS27485 (B7D44_27765) 126125..126397 - 273 WP_004182067.1 hypothetical protein -
B7D44_RS27490 (B7D44_27770) 127093..127215 - 123 WP_085842394.1 Hok/Gef family protein -
B7D44_RS27495 127163..127312 - 150 Protein_152 DUF5431 family protein -
B7D44_RS27500 (B7D44_27775) 127594..128079 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
B7D44_RS27505 (B7D44_27780) 128067..128333 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
B7D44_RS27510 (B7D44_27785) 128632..128955 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
B7D44_RS27515 (B7D44_27790) 128957..129670 + 714 WP_012540184.1 arsenical resistance protein ArsH -
B7D44_RS27520 (B7D44_27795) 129679..130224 + 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
B7D44_RS27525 (B7D44_27800) 130300..130662 + 363 WP_094716534.1 arsenic metallochaperone ArsD family protein -
B7D44_RS27530 (B7D44_27805) 130683..132440 + 1758 WP_049010932.1 arsenical pump-driving ATPase -
B7D44_RS27535 (B7D44_27810) 132565..133101 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / dfrA14 / qnrB1 / tet(A) / dfrA12 / aadA2 / qacE / sul1 / mph(A) / sul2 - 1..159323 159323


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T138557 WP_013087172.1 NZ_CP043380:c128079-127594 [Klebsiella pneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T138557 NZ_CP043380:c128079-127594 [Klebsiella pneumoniae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT138557 WP_012540086.1 NZ_CP043380:c128333-128067 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT138557 NZ_CP043380:c128333-128067 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCCAAGGAATCACAGCGGGCGCTCATTGACGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAATGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References