Detailed information of TA system TA138556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 10116..10759
• Replicon: plasmid pOXA1_095005
• Accession: NZ_CP043380
• Organism: Klebsiella pneumoniae strain WCHKP095005

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B7D44_RS26780
• Protein ID: WP_021312475.1
• Coordinates: 10343..10759 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: B7D44_RS26775
• Protein ID: WP_021312476.1
• Coordinates: 10116..10346 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D44_RS26760 (B7D44_26970)	6215..7303	+	1089	WP_094716522.1	transcriptional regulator	-
B7D44_RS26765 (B7D44_26975)	7305..8174	+	870	WP_094716519.1	hypothetical protein	-
B7D44_RS26770 (B7D44_26980)	8231..9796	-	1566	WP_094716520.1	AAA family ATPase	-
B7D44_RS26775 (B7D44_26985)	10116..10346	+	231	WP_021312476.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B7D44_RS26780 (B7D44_26990)	10343..10759	+	417	WP_021312475.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D44_RS26785 (B7D44_26995)	10794..11039	+	246	WP_065905238.1	hypothetical protein	-
B7D44_RS26790 (B7D44_27000)	11103..11321	+	219	WP_021312527.1	hypothetical protein	-
B7D44_RS26795 (B7D44_27005)	11400..11726	+	327	WP_124989605.1	hypothetical protein	-
B7D44_RS26800 (B7D44_27010)	11965..12222	+	258	WP_088401882.1	hypothetical protein	-
B7D44_RS26805 (B7D44_27015)	12222..12797	+	576	WP_065808008.1	hypothetical protein	-
B7D44_RS26810 (B7D44_27020)	12828..13322	+	495	WP_065808010.1	DNA-binding protein	-
B7D44_RS26815 (B7D44_27025)	13424..13711	-	288	WP_042938777.1	chorismate mutase	-
B7D44_RS26820 (B7D44_27030)	13866..14696	-	831	WP_226324355.1	hypothetical protein	-
B7D44_RS26825 (B7D44_27035)	15022..15288	+	267	Protein_18	ISNCY family transposase	-
B7D44_RS26830 (B7D44_27040)	15378..15473	+	96	Protein_19	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-1 / aac(6')-Ib-cr / dfrA14 / qnrB1 / tet(A) / dfrA12 / aadA2 / qacE / sul1 / mph(A) / sul2	-	1..159323	159323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15033.44 Da
• Isoelectric Point: 7.8915

>T138556 WP_021312475.1 NZ_CP043380:10343-10759 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T138556 NZ_CP043380:10343-10759 [Klebsiella pneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGTCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAATAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT138556 WP_021312476.1 NZ_CP043380:10116-10346 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT138556 NZ_CP043380:10116-10346 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4