Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5341851..5342476 Replicon chromosome
Accession NZ_CP043378
Organism Klebsiella pneumoniae strain WCHKP095005

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag B7D44_RS26075 Protein ID WP_002882817.1
Coordinates 5341851..5342234 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag B7D44_RS26080 Protein ID WP_004150355.1
Coordinates 5342234..5342476 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D44_RS26060 (5339217) 5339217..5340119 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
B7D44_RS26065 (5340116) 5340116..5340751 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
B7D44_RS26070 (5340748) 5340748..5341677 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
B7D44_RS26075 (5341851) 5341851..5342234 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
B7D44_RS26080 (5342234) 5342234..5342476 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
B7D44_RS26085 (5342681) 5342681..5343598 + 918 WP_021466676.1 alpha/beta hydrolase -
B7D44_RS26090 (5343612) 5343612..5344553 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
B7D44_RS26095 (5344598) 5344598..5345035 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
B7D44_RS26100 (5345032) 5345032..5345892 - 861 WP_004146232.1 virulence factor BrkB family protein -
B7D44_RS26105 (5345886) 5345886..5346485 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T138555 WP_002882817.1 NZ_CP043378:c5342234-5341851 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T138555 NZ_CP058153:c1109884-1109666 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
AATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTTTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT138555 WP_004150355.1 NZ_CP043378:c5342476-5342234 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT138555 NZ_CP058153:c1110286-1109912 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References