Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1468669..1469405 Replicon chromosome
Accession NZ_CP043378
Organism Klebsiella pneumoniae strain WCHKP095005

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag B7D44_RS07255 Protein ID WP_032433360.1
Coordinates 1468923..1469405 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag B7D44_RS07250 Protein ID WP_003026799.1
Coordinates 1468669..1468935 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D44_RS07225 (1464315) 1464315..1465454 + 1140 WP_032433368.1 mannitol dehydrogenase -
B7D44_RS07230 (1465483) 1465483..1466145 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
B7D44_RS07235 (1466129) 1466129..1467133 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
B7D44_RS07240 (1467151) 1467151..1467783 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
B7D44_RS07245 (1467793) 1467793..1468356 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
B7D44_RS07250 (1468669) 1468669..1468935 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
B7D44_RS07255 (1468923) 1468923..1469405 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
B7D44_RS07260 (1469766) 1469766..1470365 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
B7D44_RS07265 (1470578) 1470578..1471522 - 945 WP_077254249.1 fimbrial protein -
B7D44_RS07270 (1471534) 1471534..1472112 - 579 WP_032432061.1 type 1 fimbrial protein -
B7D44_RS07275 (1472116) 1472116..1472856 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1438892..1480348 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T138541 WP_032433360.1 NZ_CP043378:1468923-1469405 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T138541 NZ_CP043378:1468923-1469405 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT138541 WP_003026799.1 NZ_CP043378:1468669-1468935 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT138541 NZ_CP058149:2709672-2709873 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References