Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1451978..1452621 Replicon chromosome
Accession NZ_CP043378
Organism Klebsiella pneumoniae strain WCHKP095005

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag B7D44_RS07160 Protein ID WP_032433387.1
Coordinates 1452205..1452621 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag B7D44_RS07155 Protein ID WP_001261276.1
Coordinates 1451978..1452208 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D44_RS07140 (1447000) 1447000..1448087 + 1088 Protein_1395 transcriptional repressor PifC -
B7D44_RS07145 (1448090) 1448090..1450330 + 2241 WP_045326788.1 P-loop NTPase fold protein -
B7D44_RS07150 (1450858) 1450858..1451673 - 816 WP_032433388.1 hypothetical protein -
B7D44_RS07155 (1451978) 1451978..1452208 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B7D44_RS07160 (1452205) 1452205..1452621 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
B7D44_RS07165 (1452777) 1452777..1453757 + 981 WP_032433385.1 hypothetical protein -
B7D44_RS07170 (1453952) 1453952..1455523 - 1572 WP_032433383.1 AAA family ATPase -
B7D44_RS07175 (1455842) 1455842..1456090 + 249 WP_032433382.1 hypothetical protein -
B7D44_RS07180 (1456149) 1456149..1456667 + 519 WP_045326794.1 hypothetical protein -
B7D44_RS07185 (1456698) 1456698..1457189 + 492 WP_032433378.1 hypothetical protein -
B7D44_RS07190 (1457249) 1457249..1457452 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1438892..1480348 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T138540 WP_032433387.1 NZ_CP043378:1452205-1452621 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T138540 NZ_CP058149:c2256055-2255570 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT138540 WP_001261276.1 NZ_CP043378:1451978-1452208 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT138540 NZ_CP058149:c2256309-2256043 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTCGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References