Detailed information of TA system TA138535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 15596..16121
• Replicon: plasmid pCTXM15_085104
• Accession: NZ_CP043364
• Organism: Klebsiella pneumoniae strain WCHKP085104

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9GHC5
• Locus tag: B7D52_RS25870
• Protein ID: WP_009309918.1
• Coordinates: 15596..15901 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: B7D52_RS25875
• Protein ID: WP_004197642.1
• Coordinates: 15903..16121 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D52_RS25835 (B7D52_26100)	10758..11726	+	969	WP_072196505.1	IS5 family transposase	-
B7D52_RS25840	12258..12371	+	114	WP_014343462.1	hypothetical protein	-
B7D52_RS25845 (B7D52_26105)	12500..12757	+	258	WP_009310077.1	hypothetical protein	-
B7D52_RS25850 (B7D52_26110)	12815..13594	-	780	WP_023287113.1	site-specific integrase	-
B7D52_RS25855 (B7D52_26115)	13792..14808	-	1017	WP_009309921.1	hypothetical protein	-
B7D52_RS25860 (B7D52_26120)	14842..15177	-	336	WP_009309920.1	hypothetical protein	-
B7D52_RS25865	15227..15367	-	141	WP_162898808.1	hypothetical protein	-
B7D52_RS25870 (B7D52_26125)	15596..15901	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	Toxin
B7D52_RS25875 (B7D52_26130)	15903..16121	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
B7D52_RS25880 (B7D52_26145)	16720..16950	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
B7D52_RS25885 (B7D52_26150)	16947..17363	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	-
B7D52_RS25890 (B7D52_26155)	17404..18282	-	879	WP_064182004.1	restriction endonuclease	-
B7D52_RS25895 (B7D52_26160)	18944..20221	+	1278	WP_009309915.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-15 / blaTEM-1C	-	1..127885	127885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4661

>T138535 WP_009309918.1 NZ_CP043364:c15901-15596 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T138535 NZ_CP043364:c15901-15596 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT138535 WP_004197642.1 NZ_CP043364:c16121-15903 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT138535 NZ_CP043364:c16121-15903 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7H9GHC5