Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7821..8464 Replicon plasmid pKPC2_085104
Accession NZ_CP043363
Organism Klebsiella pneumoniae strain WCHKP085104

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag B7D52_RS25080 Protein ID WP_001044770.1
Coordinates 7821..8237 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag B7D52_RS25085 Protein ID WP_001261282.1
Coordinates 8234..8464 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D52_RS25050 (B7D52_25235) 2986..3673 + 688 Protein_3 IS3-like element ISEc52 family transposase -
B7D52_RS25055 (B7D52_25245) 4346..5854 + 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
B7D52_RS25060 (B7D52_25250) 5935..6114 + 180 Protein_5 DDE-type integrase/transposase/recombinase -
B7D52_RS25065 (B7D52_25255) 6396..6524 + 129 Protein_6 IS110 family transposase -
B7D52_RS25075 (B7D52_25265) 7289..7747 - 459 Protein_8 AAA family ATPase -
B7D52_RS25080 (B7D52_25270) 7821..8237 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
B7D52_RS25085 (B7D52_25275) 8234..8464 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B7D52_RS25090 (B7D52_25280) 8421..8648 + 228 Protein_11 hypothetical protein -
B7D52_RS25095 (B7D52_25290) 8975..9427 - 453 WP_004201034.1 hypothetical protein -
B7D52_RS25100 (B7D52_25300) 9846..10091 + 246 Protein_13 hypothetical protein -
B7D52_RS25105 (B7D52_25305) 10252..11196 + 945 WP_011977810.1 hypothetical protein -
B7D52_RS25110 (B7D52_25310) 11233..11625 + 393 WP_011977811.1 hypothetical protein -
B7D52_RS25115 (B7D52_25315) 11683..12204 + 522 WP_013214008.1 hypothetical protein -
B7D52_RS25120 (B7D52_25320) 12250..12453 + 204 WP_011977813.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..131027 131027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T138534 WP_001044770.1 NZ_CP043363:c8237-7821 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T138534 NZ_CP058146:c4195142-4194801 [Enterobacter hormaechei]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT138534 WP_001261282.1 NZ_CP043363:c8464-8234 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT138534 NZ_CP043363:c8464-8234 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References