Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5090190..5090815 Replicon chromosome
Accession NZ_CP043362
Organism Klebsiella pneumoniae strain WCHKP085104

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag B7D52_RS24670 Protein ID WP_002882817.1
Coordinates 5090190..5090573 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag B7D52_RS24675 Protein ID WP_004150355.1
Coordinates 5090573..5090815 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D52_RS24655 (5087556) 5087556..5088458 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
B7D52_RS24660 (5088455) 5088455..5089090 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
B7D52_RS24665 (5089087) 5089087..5090016 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
B7D52_RS24670 (5090190) 5090190..5090573 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
B7D52_RS24675 (5090573) 5090573..5090815 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
B7D52_RS24680 (5091020) 5091020..5091937 + 918 WP_004150353.1 alpha/beta hydrolase -
B7D52_RS24685 (5091951) 5091951..5092892 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
B7D52_RS24690 (5092937) 5092937..5093374 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
B7D52_RS24695 (5093371) 5093371..5094231 - 861 WP_004146232.1 virulence factor BrkB family protein -
B7D52_RS24700 (5094225) 5094225..5094824 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T138533 WP_002882817.1 NZ_CP043362:c5090573-5090190 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T138533 NZ_CP058146:c3939179-3938769 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT138533 WP_004150355.1 NZ_CP043362:c5090815-5090573 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT138533 NZ_CP043362:c5090815-5090573 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References