Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3894721..3895340 Replicon chromosome
Accession NZ_CP043362
Organism Klebsiella pneumoniae strain WCHKP085104

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B7D52_RS19045 Protein ID WP_002892050.1
Coordinates 3895122..3895340 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag B7D52_RS19040 Protein ID WP_002892066.1
Coordinates 3894721..3895095 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D52_RS19030 (3889873) 3889873..3891066 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B7D52_RS19035 (3891089) 3891089..3894235 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
B7D52_RS19040 (3894721) 3894721..3895095 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
B7D52_RS19045 (3895122) 3895122..3895340 + 219 WP_002892050.1 HHA domain-containing protein Toxin
B7D52_RS19050 (3895499) 3895499..3896065 + 567 WP_002892030.1 maltose O-acetyltransferase -
B7D52_RS19055 (3896037) 3896037..3896177 - 141 WP_004147370.1 hypothetical protein -
B7D52_RS19060 (3896198) 3896198..3896668 + 471 WP_002892026.1 YlaC family protein -
B7D52_RS19065 (3896643) 3896643..3898094 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
B7D52_RS19070 (3898195) 3898195..3898893 + 699 WP_002892021.1 GNAT family protein -
B7D52_RS19075 (3898890) 3898890..3899030 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
B7D52_RS19080 (3899030) 3899030..3899293 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138529 WP_002892050.1 NZ_CP043362:3895122-3895340 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T138529 NZ_CP058146:c396827-396540 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT138529 WP_002892066.1 NZ_CP043362:3894721-3895095 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT138529 NZ_CP058146:c397066-396827 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References