Detailed information of TA system TA138516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1239..1713
• Replicon: plasmid p3_020120
• Accession: NZ_CP043361
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: CLQ78_RS29065
• Protein ID: WP_004197762.1
• Coordinates: 1239..1505 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: CLQ78_RS29070
• Protein ID: WP_000879771.1
• Coordinates: 1489..1713 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ78_RS29055 (CLQ78_29035)	208..753	+	546	WP_023317533.1	hypothetical protein	-
CLQ78_RS29060 (CLQ78_29040)	819..1169	+	351	WP_004197756.1	hypothetical protein	-
CLQ78_RS29065 (CLQ78_29045)	1239..1505	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ78_RS29070 (CLQ78_29050)	1489..1713	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
CLQ78_RS29075 (CLQ78_29055)	2380..2592	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
CLQ78_RS29080 (CLQ78_29060)	2599..3108	-	510	WP_032433972.1	MbeB family mobilization protein	-
CLQ78_RS29450	3143..3577	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
CLQ78_RS29090 (CLQ78_29070)	3786..4130	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
CLQ78_RS29095 (CLQ78_29075)	4120..4296	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T138516 WP_004197762.1 NZ_CP043361:c1505-1239 [Klebsiella pneumoniae subsp. pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T138516 NZ_CP058131:821989-822399 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGAATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGGGACCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT138516 WP_000879771.1 NZ_CP043361:c1713-1489 [Klebsiella pneumoniae subsp. pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT138516 NZ_CP043361:c1713-1489 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5