Detailed information of TA system TA138515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2346..2926
• Replicon: plasmid p2_020120
• Accession: NZ_CP043360
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CLQ78_RS29010
• Protein ID: WP_071177730.1
• Coordinates: 2346..2660 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CLQ78_RS29015
• Protein ID: WP_000093040.1
• Coordinates: 2648..2926 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ78_RS28990 (CLQ78_28970)	254..985	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
CLQ78_RS28995 (CLQ78_28975)	992..1522	+	531	WP_071177729.1	hypothetical protein	-
CLQ78_RS29000 (CLQ78_28980)	1549..1728	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CLQ78_RS29005 (CLQ78_28985)	1754..2182	-	429	WP_001140599.1	hypothetical protein	-
CLQ78_RS29010 (CLQ78_28990)	2346..2660	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ78_RS29015 (CLQ78_28995)	2648..2926	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CLQ78_RS29370	3101..3466	-	366	WP_072354022.1	TonB family protein	-
CLQ78_RS29025 (CLQ78_29005)	3463..3834	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
CLQ78_RS29030 (CLQ78_29010)	4108..4353	-	246	WP_032440458.1	hypothetical protein	-
CLQ78_RS29035 (CLQ78_29015)	4680..6365	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
CLQ78_RS29040 (CLQ78_29020)	6375..6632	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
CLQ78_RS29045 (CLQ78_29025)	6717..6866	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T138515 WP_071177730.1 NZ_CP043360:c2660-2346 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T138515 NZ_CP058129:c86206-85721 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT138515 WP_000093040.1 NZ_CP043360:c2926-2648 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT138515 NZ_CP043360:c2926-2648 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1