Detailed information of TA system TA138514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 182883..183526
• Replicon: plasmid p1_020120
• Accession: NZ_CP043359
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CLQ78_RS28865
• Protein ID: WP_014386165.1
• Coordinates: 183110..183526 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CLQ78_RS28860
• Protein ID: WP_032408893.1
• Coordinates: 182883..183113 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ78_RS28835 (CLQ78_28815)	177956..178939	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
CLQ78_RS28840 (CLQ78_28820)	178958..180106	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
CLQ78_RS28845 (CLQ78_28825)	180277..181434	-	1158	WP_032408895.1	acetylornithine deacetylase	-
CLQ78_RS28850 (CLQ78_28830)	181450..182124	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
CLQ78_RS28855 (CLQ78_28835)	182129..182563	-	435	WP_000103648.1	RidA family protein	-
CLQ78_RS28860 (CLQ78_28840)	182883..183113	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CLQ78_RS28865 (CLQ78_28845)	183110..183526	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
CLQ78_RS28870 (CLQ78_28850)	183849..184817	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
CLQ78_RS28875 (CLQ78_28855)	184997..185371	-	375	WP_032408891.1	hypothetical protein	-
CLQ78_RS28880 (CLQ78_28860)	185427..185753	-	327	WP_004152639.1	hypothetical protein	-
CLQ78_RS28885 (CLQ78_28865)	185750..186478	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
CLQ78_RS28890 (CLQ78_28870)	186475..186906	-	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205652	205652
	-	flank	IS/Tn	-	-	183849..184817	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T138514 WP_014386165.1 NZ_CP043359:183110-183526 [Klebsiella pneumoniae subsp. pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T138514 NZ_CP043359:183110-183526 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT138514 WP_032408893.1 NZ_CP043359:182883-183113 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT138514 NZ_CP058129:c32296-32066 [Enterobacter hormaechei]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA