Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 53667..54403 | Replicon | plasmid p1_020120 |
| Accession | NZ_CP043359 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | L7SZ15 |
| Locus tag | CLQ78_RS28140 | Protein ID | WP_003026803.1 |
| Coordinates | 53921..54403 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | L7SZ29 |
| Locus tag | CLQ78_RS28135 | Protein ID | WP_003026799.1 |
| Coordinates | 53667..53933 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CLQ78_RS28090 (CLQ78_28070) | 49729..50091 | - | 363 | WP_004152100.1 | arsenite efflux transporter metallochaperone ArsD | - |
| CLQ78_RS28095 (CLQ78_28075) | 50141..50491 | - | 351 | WP_004152101.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| CLQ78_RS28100 (CLQ78_28080) | 50849..51118 | + | 270 | WP_004152102.1 | hypothetical protein | - |
| CLQ78_RS28105 (CLQ78_28085) | 51106..51681 | + | 576 | WP_004152103.1 | hypothetical protein | - |
| CLQ78_RS28110 (CLQ78_28090) | 51712..52206 | + | 495 | WP_004152104.1 | DNA-binding protein | - |
| CLQ78_RS28115 (CLQ78_28095) | 52250..52618 | + | 369 | WP_004152105.1 | hypothetical protein | - |
| CLQ78_RS28120 (CLQ78_28100) | 52652..52855 | + | 204 | WP_004152106.1 | HHA domain-containing protein | - |
| CLQ78_RS28125 (CLQ78_28105) | 52904..53161 | + | 258 | WP_004152107.1 | hypothetical protein | - |
| CLQ78_RS28130 (CLQ78_28110) | 53237..53491 | + | 255 | WP_004152108.1 | hypothetical protein | - |
| CLQ78_RS28135 (CLQ78_28115) | 53667..53933 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
| CLQ78_RS28140 (CLQ78_28120) | 53921..54403 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
| CLQ78_RS28145 (CLQ78_28125) | 54615..55961 | + | 1347 | WP_020314316.1 | ISNCY family transposase | - |
| CLQ78_RS29445 | 56122..56253 | + | 132 | WP_004218042.1 | hypothetical protein | - |
| CLQ78_RS28150 (CLQ78_28130) | 56462..57627 | - | 1166 | Protein_59 | IS3 family transposase | - |
| CLQ78_RS28155 (CLQ78_28135) | 57804..58766 | - | 963 | WP_004152113.1 | zinc metalloprotease HtpX | - |
| CLQ78_RS28160 (CLQ78_28140) | 58753..59241 | - | 489 | WP_004152114.1 | phosphate-starvation-inducible PsiE family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A) | - | 1..205652 | 205652 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T138512 WP_003026803.1 NZ_CP043359:53921-54403 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T138512 NZ_CP058128:c4120190-4119849 [Enterobacter hormaechei]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT138512 WP_003026799.1 NZ_CP043359:53667-53933 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT138512 NZ_CP043359:53667-53933 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2GNW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YL66 |