Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 140411..141054 | Replicon | plasmid pKPC2_020120 |
| Accession | NZ_CP043358 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | CLQ78_RS27780 | Protein ID | WP_001044770.1 |
| Coordinates | 140638..141054 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | CLQ78_RS27775 | Protein ID | WP_001261282.1 |
| Coordinates | 140411..140641 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CLQ78_RS27740 (135634) | 135634..136413 | - | 780 | WP_013214009.1 | site-specific integrase | - |
| CLQ78_RS27745 (136597) | 136597..137601 | - | 1005 | WP_011977814.1 | hypothetical protein | - |
| CLQ78_RS27750 (137631) | 137631..137834 | - | 204 | WP_011977813.1 | hypothetical protein | - |
| CLQ78_RS27755 (137880) | 137880..138401 | - | 522 | WP_013214008.1 | hypothetical protein | - |
| CLQ78_RS27760 (138459) | 138459..138851 | - | 393 | WP_011977811.1 | hypothetical protein | - |
| CLQ78_RS27765 (138888) | 138888..139832 | - | 945 | WP_011977810.1 | hypothetical protein | - |
| CLQ78_RS27770 (139993) | 139993..140454 | - | 462 | WP_014343465.1 | hypothetical protein | - |
| CLQ78_RS27775 (140411) | 140411..140641 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| CLQ78_RS27780 (140638) | 140638..141054 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| CLQ78_RS27785 (141128) | 141128..142690 | + | 1563 | WP_004206609.1 | AAA family ATPase | - |
| CLQ78_RS27790 (142675) | 142675..143697 | + | 1023 | WP_000361404.1 | helicase UvrD | - |
| CLQ78_RS27795 (143953) | 143953..144650 | + | 698 | WP_223174630.1 | IS1 family transposase | - |
| CLQ78_RS27800 (144679) | 144679..144951 | + | 273 | Protein_190 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB | - | 1..154719 | 154719 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T138509 WP_001044770.1 NZ_CP043358:140638-141054 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T138509 NZ_CP058128:c1258174-1257863 [Enterobacter hormaechei]
ATGCCCGCAGTCTCAAGAGCGCCTTTCGACGCGGCCACCAGACATTTTCCTATTCAAGAACTCGCTCTGGAAGATCTTTA
CTGGGTACTAAAAGGAGAGCACTACCCATCACCAGACGACATGCGAAAGCGCTTCCCCAGCCGGGAGAGGATGAAATACC
GGGAAAAATGGTGGGTCATTGACGTAGGTGGCGGGTATTTAAGAGTAATGTTATTTACTGATTTTGAGCGCGGAAAGCTC
TTCATCAAACACACCAGCACTCACGCAGATTATGACAAGCTCACGGACCTCTACCGGAGAAAGAAAGAATGA
ATGCCCGCAGTCTCAAGAGCGCCTTTCGACGCGGCCACCAGACATTTTCCTATTCAAGAACTCGCTCTGGAAGATCTTTA
CTGGGTACTAAAAGGAGAGCACTACCCATCACCAGACGACATGCGAAAGCGCTTCCCCAGCCGGGAGAGGATGAAATACC
GGGAAAAATGGTGGGTCATTGACGTAGGTGGCGGGTATTTAAGAGTAATGTTATTTACTGATTTTGAGCGCGGAAAGCTC
TTCATCAAACACACCAGCACTCACGCAGATTATGACAAGCTCACGGACCTCTACCGGAGAAAGAAAGAATGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT138509 WP_001261282.1 NZ_CP043358:140411-140641 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT138509 NZ_CP058128:c1257866-1257447 [Enterobacter hormaechei]
ATGAACTTTTCAGCAGCCATCAAAGCGGCAAACGATCTAACAAGGGAGCTCCCTTTCCTGGGCTCAAGCCCGTCGCGACA
AGATTATGAGGATGCTATGGGGTTGGTGGAGTATCTAATTGAGCATGACCCCGATAGTCCGCTGGTAGAGATGCTCACCG
CTAAAATCGATAAATACGAAAACGAGTCTCCGGAGTTTGCAGAATTTAACGCGCGTGTTGCCAGCATTCCGTCAGGCGTT
GCGTTATTGCGAACGCTGATGGATCAGTACCATCTCACTCAGAGCGATTTCGAAAACGAAATAGGTAAGAAGTCTCTGGT
GAGTCGGATCCTCAACGGACAGCGCACGTTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGGCGTGCAGGTAA
GTGCATTTGTGGGGAATTGA
ATGAACTTTTCAGCAGCCATCAAAGCGGCAAACGATCTAACAAGGGAGCTCCCTTTCCTGGGCTCAAGCCCGTCGCGACA
AGATTATGAGGATGCTATGGGGTTGGTGGAGTATCTAATTGAGCATGACCCCGATAGTCCGCTGGTAGAGATGCTCACCG
CTAAAATCGATAAATACGAAAACGAGTCTCCGGAGTTTGCAGAATTTAACGCGCGTGTTGCCAGCATTCCGTCAGGCGTT
GCGTTATTGCGAACGCTGATGGATCAGTACCATCTCACTCAGAGCGATTTCGAAAACGAAATAGGTAAGAAGTCTCTGGT
GAGTCGGATCCTCAACGGACAGCGCACGTTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGGCGTGCAGGTAA
GTGCATTTGTGGGGAATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |