Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5384144..5384769 Replicon chromosome
Accession NZ_CP043357
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CLQ78_RS26510 Protein ID WP_002882817.1
Coordinates 5384144..5384527 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CLQ78_RS26515 Protein ID WP_004150355.1
Coordinates 5384527..5384769 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ78_RS26495 (CLQ78_26475) 5381510..5382412 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CLQ78_RS26500 (CLQ78_26480) 5382409..5383044 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CLQ78_RS26505 (CLQ78_26485) 5383041..5383970 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CLQ78_RS26510 (CLQ78_26490) 5384144..5384527 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ78_RS26515 (CLQ78_26495) 5384527..5384769 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CLQ78_RS26520 (CLQ78_26500) 5384974..5385891 + 918 WP_002882812.1 alpha/beta hydrolase -
CLQ78_RS26525 (CLQ78_26505) 5385905..5386846 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CLQ78_RS26530 (CLQ78_26510) 5386891..5387328 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CLQ78_RS26535 (CLQ78_26515) 5387325..5388185 - 861 WP_002882807.1 virulence factor BrkB family protein -
CLQ78_RS26540 (CLQ78_26520) 5388179..5388778 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T138505 WP_002882817.1 NZ_CP043357:c5384527-5384144 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T138505 NZ_CP058120:5079-5561 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT138505 WP_004150355.1 NZ_CP043357:c5384769-5384527 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT138505 NZ_CP043357:c5384769-5384527 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References