Detailed information of TA system TA138505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5384144..5384769
• Replicon: chromosome
• Accession: NZ_CP043357
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: CLQ78_RS26510
• Protein ID: WP_002882817.1
• Coordinates: 5384144..5384527 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: CLQ78_RS26515
• Protein ID: WP_004150355.1
• Coordinates: 5384527..5384769 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ78_RS26495 (CLQ78_26475)	5381510..5382412	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
CLQ78_RS26500 (CLQ78_26480)	5382409..5383044	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
CLQ78_RS26505 (CLQ78_26485)	5383041..5383970	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
CLQ78_RS26510 (CLQ78_26490)	5384144..5384527	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
CLQ78_RS26515 (CLQ78_26495)	5384527..5384769	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
CLQ78_RS26520 (CLQ78_26500)	5384974..5385891	+	918	WP_002882812.1	alpha/beta hydrolase	-
CLQ78_RS26525 (CLQ78_26505)	5385905..5386846	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
CLQ78_RS26530 (CLQ78_26510)	5386891..5387328	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
CLQ78_RS26535 (CLQ78_26515)	5387325..5388185	-	861	WP_002882807.1	virulence factor BrkB family protein	-
CLQ78_RS26540 (CLQ78_26520)	5388179..5388778	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T138505 WP_002882817.1 NZ_CP043357:c5384527-5384144 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T138505 NZ_CP058120:5079-5561 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT138505 WP_004150355.1 NZ_CP043357:c5384769-5384527 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT138505 NZ_CP043357:c5384769-5384527 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9