138502

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4801294..4802104 Replicon chromosome
Accession NZ_CP043357
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag CLQ78_RS23725 Protein ID WP_002887280.1
Coordinates 4801294..4801827 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag CLQ78_RS23730 Protein ID WP_002887278.1
Coordinates 4801838..4802104 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ78_RS23720 (CLQ78_23710) 4800125..4801246 + 1122 WP_002887282.1 cupin domain-containing protein -
CLQ78_RS23725 (CLQ78_23715) 4801294..4801827 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
CLQ78_RS23730 (CLQ78_23720) 4801838..4802104 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
CLQ78_RS23735 (CLQ78_23725) 4802207..4803640 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
CLQ78_RS23740 (CLQ78_23730) 4803630..4804313 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
CLQ78_RS23745 (CLQ78_23735) 4804485..4805870 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
CLQ78_RS23750 (CLQ78_23740) 4805888..4806232 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T138502 WP_002887280.1 NZ_CP043357:c4801827-4801294 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T138502 NZ_CP058119:c78325-78038 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAGCTGGATAAGTCCATTCAGCAGCAGTTTGC
GAAGAAACTGAAGAAATGCTGTGACGAACCACATATCCCCTCTGCAAAGCTGAGAGGAATAAAGGACTGTTATAAAATTA
AACTTCGTGCATCAGGTTTTCGCCTGGTGTATCAGGTGATAGATGATCAATTAATTATCGCGGTTGTTGCTGTTGGAAAG
CGTGAACGCAGTGACGTGTACAATCTGGCCAGCGAAAGGATGAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT138502 WP_002887278.1 NZ_CP043357:c4802104-4801838 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT138502 NZ_CP043357:c4802104-4801838 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References