Detailed information of TA system TA138487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1680..2154
• Replicon: plasmid p2_020115
• Accession: NZ_CP043356
• Organism: Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: C6M09_RS27870
• Protein ID: WP_004197762.1
• Coordinates: 1680..1946 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: C6M09_RS27875
• Protein ID: WP_000879771.1
• Coordinates: 1930..2154 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M09_RS27855 (C6M09_27835)	297..623	+	327	WP_050516389.1	hypothetical protein	-
C6M09_RS27860 (C6M09_27840)	649..1194	+	546	WP_023317533.1	hypothetical protein	-
C6M09_RS27865 (C6M09_27845)	1260..1610	+	351	WP_004197756.1	hypothetical protein	-
C6M09_RS27870 (C6M09_27850)	1680..1946	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6M09_RS27875 (C6M09_27855)	1930..2154	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
C6M09_RS27880 (C6M09_27860)	2821..3033	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
C6M09_RS27885 (C6M09_27865)	3040..3549	-	510	WP_032433972.1	MbeB family mobilization protein	-
C6M09_RS28205	3584..4018	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
C6M09_RS27895 (C6M09_27875)	4227..4571	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
C6M09_RS27900 (C6M09_27880)	4561..4737	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T138487 WP_004197762.1 NZ_CP043356:c1946-1680 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T138487 NZ_CP058112:2014040-2014393 [Klebsiella grimontii]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGTTCAACGATACCGATAGGGCCAGGGTGCTTGC
AGCGTTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTAATGCCGATACGCTGAGAGGCTCCCGCTACAGCA
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGTAATAAGGTTGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGAGCGGGAATTCAC
GAACTGGCTGAAGACATTCAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT138487 WP_000879771.1 NZ_CP043356:c2154-1930 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT138487 NZ_CP058112:2014398-2014700 [Klebsiella grimontii]
ATGGGCAGAACGTTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAGATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCGGATGGTTCTCACTACGGATTTGAACTGTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5