Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68235..68878 Replicon plasmid p1_020115
Accession NZ_CP043355
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C6M09_RS27790 Protein ID WP_001044770.1
Coordinates 68235..68651 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C6M09_RS27795 Protein ID WP_001261282.1
Coordinates 68648..68878 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS27770 (C6M09_27750) 64338..64610 - 273 Protein_87 transposase -
C6M09_RS27780 (C6M09_27760) 65592..66614 - 1023 WP_000361404.1 helicase UvrD -
C6M09_RS27785 (C6M09_27765) 66599..68161 - 1563 WP_004206609.1 AAA family ATPase -
C6M09_RS27790 (C6M09_27770) 68235..68651 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M09_RS27795 (C6M09_27775) 68648..68878 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
C6M09_RS27800 (C6M09_27780) 68835..69296 + 462 WP_014343465.1 hypothetical protein -
C6M09_RS27805 (C6M09_27785) 69457..70401 + 945 WP_011977810.1 hypothetical protein -
C6M09_RS27810 (C6M09_27790) 70438..70830 + 393 WP_011977811.1 hypothetical protein -
C6M09_RS27815 (C6M09_27795) 70888..71409 + 522 WP_013214008.1 hypothetical protein -
C6M09_RS27820 (C6M09_27800) 71455..71658 + 204 WP_011977813.1 hypothetical protein -
C6M09_RS27825 (C6M09_27805) 71688..72692 + 1005 WP_011977814.1 hypothetical protein -
C6M09_RS27830 (C6M09_27810) 72876..73655 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA16 / dfrA27 / ARR-3 - 1..78836 78836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T138486 WP_001044770.1 NZ_CP043355:c68651-68235 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T138486 NZ_CP058112:940274-940684 [Klebsiella grimontii]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCATGGCGCGCGCAGTGGTTTTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTAGTGTTCGACTGCG
TACGCAGCCAGCGGCGGATCCACGCCCGTCAGGGCGAAATAAAGCTGCTGATCGATTCGCGTCTGCGCTGGCAAAAGGCG
GAGTGGGAGATTATCGGTACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT138486 WP_001261282.1 NZ_CP043355:c68878-68648 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT138486 NZ_CP043355:c68878-68648 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References