Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5367822..5368447 Replicon chromosome
Accession NZ_CP043353
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag C6M09_RS26390 Protein ID WP_002882817.1
Coordinates 5367822..5368205 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C6M09_RS26395 Protein ID WP_004150355.1
Coordinates 5368205..5368447 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS26375 (C6M09_26355) 5365188..5366090 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C6M09_RS26380 (C6M09_26360) 5366087..5366722 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C6M09_RS26385 (C6M09_26365) 5366719..5367648 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C6M09_RS26390 (C6M09_26370) 5367822..5368205 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M09_RS26395 (C6M09_26375) 5368205..5368447 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
C6M09_RS26400 (C6M09_26380) 5368652..5369569 + 918 WP_002882812.1 alpha/beta hydrolase -
C6M09_RS26405 (C6M09_26385) 5369583..5370524 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C6M09_RS26410 (C6M09_26390) 5370569..5371006 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
C6M09_RS26415 (C6M09_26395) 5371003..5371863 - 861 WP_002882807.1 virulence factor BrkB family protein -
C6M09_RS26420 (C6M09_26400) 5371857..5372456 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T138481 WP_002882817.1 NZ_CP043353:c5368205-5367822 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T138481 NZ_CP058057:c4302933-4302781 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAATTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT138481 WP_004150355.1 NZ_CP043353:c5368447-5368205 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT138481 NZ_CP043353:c5368447-5368205 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References