Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4781894..4782704 Replicon chromosome
Accession NZ_CP043353
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag C6M09_RS23580 Protein ID WP_002887280.1
Coordinates 4781894..4782427 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag C6M09_RS23585 Protein ID WP_002887278.1
Coordinates 4782438..4782704 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS23575 (C6M09_23565) 4780725..4781846 + 1122 WP_002887282.1 cupin domain-containing protein -
C6M09_RS23580 (C6M09_23570) 4781894..4782427 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
C6M09_RS23585 (C6M09_23575) 4782438..4782704 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
C6M09_RS23590 (C6M09_23580) 4782807..4784240 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
C6M09_RS23595 (C6M09_23585) 4784230..4784913 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
C6M09_RS23600 (C6M09_23590) 4785085..4786470 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
C6M09_RS23605 (C6M09_23595) 4786488..4786832 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T138478 WP_002887280.1 NZ_CP043353:c4782427-4781894 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T138478 NZ_CP058057:c4272562-4272455 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT138478 WP_002887278.1 NZ_CP043353:c4782704-4782438 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT138478 NZ_CP058057:4272620-4272674 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References