Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4166592..4167211 Replicon chromosome
Accession NZ_CP043353
Organism Klebsiella pneumoniae strain WCHKP020115

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C6M09_RS20615 Protein ID WP_002892050.1
Coordinates 4166993..4167211 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C6M09_RS20610 Protein ID WP_002892066.1
Coordinates 4166592..4166966 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M09_RS20600 (C6M09_20590) 4161744..4162937 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C6M09_RS20605 (C6M09_20595) 4162960..4166106 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
C6M09_RS20610 (C6M09_20600) 4166592..4166966 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C6M09_RS20615 (C6M09_20605) 4166993..4167211 + 219 WP_002892050.1 HHA domain-containing protein Toxin
C6M09_RS20620 (C6M09_20610) 4167370..4167936 + 567 WP_002892030.1 maltose O-acetyltransferase -
C6M09_RS20625 (C6M09_20615) 4167908..4168048 - 141 WP_004147370.1 hypothetical protein -
C6M09_RS20630 (C6M09_20620) 4168069..4168539 + 471 WP_002892026.1 YlaC family protein -
C6M09_RS20635 (C6M09_20625) 4168514..4169965 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
C6M09_RS20640 (C6M09_20630) 4170066..4170764 + 699 WP_002892021.1 GNAT family protein -
C6M09_RS20645 (C6M09_20635) 4170761..4170901 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C6M09_RS20650 (C6M09_20640) 4170901..4171164 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138477 WP_002892050.1 NZ_CP043353:4166993-4167211 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T138477 NZ_CP043353:4166993-4167211 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT138477 WP_002892066.1 NZ_CP043353:4166592-4166966 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT138477 NZ_CP043353:4166592-4166966 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References