Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5791..6316 Replicon plasmid pCTXM15_020097
Accession NZ_CP043351
Organism Klebsiella pneumoniae strain WCHKP020097

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CAY71_RS27450 Protein ID WP_013023785.1
Coordinates 5791..6096 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CAY71_RS27455 Protein ID WP_001568025.1
Coordinates 6098..6316 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY71_RS27420 (1518) 1518..2144 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
CAY71_RS27425 (2141) 2141..2443 + 303 WP_004197636.1 hypothetical protein -
CAY71_RS27430 (2867) 2867..3661 - 795 WP_004197635.1 site-specific integrase -
CAY71_RS27435 (3859) 3859..4875 - 1017 WP_017899884.1 hypothetical protein -
CAY71_RS27440 (4886) 4886..5200 - 315 WP_053389906.1 hypothetical protein -
CAY71_RS27445 (5227) 5227..5622 - 396 WP_017899885.1 hypothetical protein -
CAY71_RS27450 (5791) 5791..6096 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CAY71_RS27455 (6098) 6098..6316 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CAY71_RS27460 (6871) 6871..7560 + 690 WP_004193995.1 hypothetical protein -
CAY71_RS27465 (7592) 7592..8281 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
CAY71_RS27470 (8718) 8718..8966 + 249 WP_000272716.1 hypothetical protein -
CAY71_RS27475 (8963) 8963..9535 + 573 WP_020805590.1 hypothetical protein -
CAY71_RS27480 (9566) 9566..10060 + 495 WP_020805591.1 hypothetical protein -
CAY71_RS27485 (10104) 10104..10472 + 369 WP_004197807.1 hypothetical protein -
CAY71_RS27490 (10506) 10506..10709 + 204 WP_004197808.1 HHA domain-containing protein -
CAY71_RS27495 (10723) 10723..10926 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / aac(3)-IId - 1..106448 106448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T138463 WP_013023785.1 NZ_CP043351:c6096-5791 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T138463 NZ_CP057919:32851-33345 [Escherichia fergusonii]
ATGGAATACTTAGAAATAAACGGTTGGAAAATCTGCTTTCACGAGTGTTTTTTAAGGCAGATTACAGGACTAATGAAGAA
GGTGGCAGAGTTAAAGGCTTCTAAACCACTGGAATATCATAAGAAAAAGGAAACTAAACTTCTTGTTGCGATCGAGCGTG
TTATAGAGGAAAGGATAACCGCTAACCCGCTTGATCCACAGTTTCGGCAGGGAAATACTTTAGGCGAAGAATACAAACAC
TGGTTCCGGGCAAAATTTTTGATGCAGTTCCGTTTGTTTTATCGTTGTAGCCAGGAACATAAAACAATTGTTATTGGCTG
GGTAAATGGATTTGACACGCTTCGTGCTTATGATTCCAGGACGGATGCCTATAAGGTATTTGGTGAAATGTTGAAAGCTG
GAAATCCACCGGATGACTGGGATACCCTTCTCTGTGAGGCTGGTAAAGCTACAGCGACCACCAATATGGTTGATTTTCTC
ACAAAGCGTATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT138463 WP_001568025.1 NZ_CP043351:c6316-6098 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT138463 NZ_CP043351:c6316-6098 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References