Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36387..37030 Replicon plasmid pKPC2_020097
Accession NZ_CP043349
Organism Klebsiella pneumoniae strain WCHKP020097

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag CAY71_RS25790 Protein ID WP_032425563.1
Coordinates 36387..36803 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag CAY71_RS25795 Protein ID WP_001261276.1
Coordinates 36800..37030 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY71_RS25775 (CAY71_26025) 31431..33539 - 2109 WP_032427226.1 peptidase domain-containing ABC transporter -
CAY71_RS25780 (CAY71_26030) 33529..34806 - 1278 WP_009309915.1 HlyD family secretion protein -
CAY71_RS25785 (CAY71_26035) 35468..36346 + 879 WP_064182004.1 restriction endonuclease -
CAY71_RS25790 (CAY71_26040) 36387..36803 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
CAY71_RS25795 (CAY71_26045) 36800..37030 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CAY71_RS25800 (CAY71_26055) 37618..38562 + 945 WP_011977810.1 hypothetical protein -
CAY71_RS25805 (CAY71_26060) 38599..38991 + 393 WP_011977811.1 hypothetical protein -
CAY71_RS25810 (CAY71_26065) 39049..39570 + 522 WP_013214008.1 hypothetical protein -
CAY71_RS25815 (CAY71_26070) 39616..39819 + 204 WP_011977813.1 hypothetical protein -
CAY71_RS25820 (CAY71_26075) 39849..40853 + 1005 WP_011977814.1 hypothetical protein -
CAY71_RS25825 (CAY71_26080) 41037..41816 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / qnrB1 / blaKPC-2 - 1..141339 141339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T138462 WP_032425563.1 NZ_CP043349:c36803-36387 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T138462 NZ_CP043349:c36803-36387 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT138462 WP_001261276.1 NZ_CP043349:c37030-36800 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT138462 NZ_CP057919:2027-2278 [Escherichia fergusonii]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References