Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4607350..4608160 Replicon chromosome
Accession NZ_CP043348
Organism Klebsiella pneumoniae strain WCHKP020097

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag CAY71_RS22365 Protein ID WP_004178461.1
Coordinates 4607350..4607883 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag CAY71_RS22370 Protein ID WP_002887278.1
Coordinates 4607894..4608160 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY71_RS22360 (4606181) 4606181..4607302 + 1122 WP_002887282.1 cupin domain-containing protein -
CAY71_RS22365 (4607350) 4607350..4607883 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
CAY71_RS22370 (4607894) 4607894..4608160 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
CAY71_RS22375 (4608263) 4608263..4609696 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
CAY71_RS22380 (4609686) 4609686..4610369 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
CAY71_RS22385 (4610541) 4610541..4611926 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
CAY71_RS22390 (4611944) 4611944..4612288 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T138457 WP_004178461.1 NZ_CP043348:c4607883-4607350 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T138457 NZ_CP057918:c2544887-2544534 [Escherichia fergusonii]
GTGTGGACGATTAAAACAACGGATATGTTTGATCACTGGTTTATCTCGCTCAACGATACCGACAGGGCCAGCGTGCTTGC
AGCGCTGTTGGTCCTGCGGGAAAAGGGGCCGGGGCTATCCAGACCTTATGCCGATACGATCAGAGGTTCCCGCTACAGCA
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGATCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACTGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCGATCGCAGACCGGGAATTCAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT138457 WP_002887278.1 NZ_CP043348:c4608160-4607894 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT138457 NZ_CP057918:c2544529-2544227 [Escherichia fergusonii]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTCGTGGACGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAACATTCATCTTGCTGAGTTGCGTGAGAAAGTACAGAAAACGCAGGTGGAAATGGCGCAGGCGCTGGGTATCA
GACAGCCTACCGTTGCAGGAATGGAAAAACCGGGCCGCGACCTGAAACTTTCCACGCTTAAACGCTACGTTGAAGCGACC
GGCGGCAAATTGCGGCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTCGTACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References