Detailed information of TA system TA138444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1364..1944
• Replicon: plasmid p1_020039
• Accession: NZ_CP043346
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CVC44_RS27340
• Protein ID: WP_071177730.1
• Coordinates: 1364..1678 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CVC44_RS27345
• Protein ID: WP_000093040.1
• Coordinates: 1666..1944 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVC44_RS27325 (CVC44_27720)	10..540	+	531	WP_071177729.1	hypothetical protein	-
CVC44_RS27330 (CVC44_27725)	567..746	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CVC44_RS27335 (CVC44_27730)	772..1200	-	429	WP_001140599.1	hypothetical protein	-
CVC44_RS27340 (CVC44_27735)	1364..1678	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CVC44_RS27345 (CVC44_27740)	1666..1944	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CVC44_RS27350 (CVC44_27745)	2119..2484	-	366	WP_072354022.1	TonB family protein	-
CVC44_RS27355 (CVC44_27750)	2481..2852	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
CVC44_RS27360 (CVC44_27755)	3126..3371	-	246	WP_032440458.1	hypothetical protein	-
CVC44_RS27365 (CVC44_27760)	3698..5383	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
CVC44_RS27370 (CVC44_27765)	5393..5650	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
CVC44_RS27375 (CVC44_27770)	5735..5884	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T138444 WP_071177730.1 NZ_CP043346:c1678-1364 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T138444 NZ_CP057896:860082-860417 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT138444 WP_000093040.1 NZ_CP043346:c1944-1666 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT138444 NZ_CP057896:859834-860082 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1