138438

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5316167..5316792 Replicon chromosome
Accession NZ_CP043344
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CVC44_RS26075 Protein ID WP_002882817.1
Coordinates 5316167..5316550 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CVC44_RS26080 Protein ID WP_004150355.1
Coordinates 5316550..5316792 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVC44_RS26060 (CVC44_26275) 5313533..5314435 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CVC44_RS26065 (CVC44_26280) 5314432..5315067 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CVC44_RS26070 (CVC44_26285) 5315064..5315993 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CVC44_RS26075 (CVC44_26290) 5316167..5316550 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CVC44_RS26080 (CVC44_26295) 5316550..5316792 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CVC44_RS26085 (CVC44_26300) 5316997..5317914 + 918 WP_002882812.1 alpha/beta hydrolase -
CVC44_RS26090 (CVC44_26305) 5317928..5318869 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CVC44_RS26095 (CVC44_26310) 5318914..5319351 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CVC44_RS26100 (CVC44_26315) 5319348..5320208 - 861 WP_002882807.1 virulence factor BrkB family protein -
CVC44_RS26105 (CVC44_26320) 5320202..5320801 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T138438 WP_002882817.1 NZ_CP043344:c5316550-5316167 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T138438 NZ_CP043344:c5316550-5316167 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT138438 WP_004150355.1 NZ_CP043344:c5316792-5316550 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT138438 NZ_CP043344:c5316792-5316550 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References