Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4731439..4732249 Replicon chromosome
Accession NZ_CP043344
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag CVC44_RS23270 Protein ID WP_002887280.1
Coordinates 4731439..4731972 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag CVC44_RS23275 Protein ID WP_002887278.1
Coordinates 4731983..4732249 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVC44_RS23265 (CVC44_23440) 4730270..4731391 + 1122 WP_002887282.1 cupin domain-containing protein -
CVC44_RS23270 (CVC44_23445) 4731439..4731972 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
CVC44_RS23275 (CVC44_23450) 4731983..4732249 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
CVC44_RS23280 (CVC44_23455) 4732352..4733785 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
CVC44_RS23285 (CVC44_23460) 4733775..4734458 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
CVC44_RS23290 (CVC44_23465) 4734630..4736015 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
CVC44_RS23295 (CVC44_23470) 4736033..4736377 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T138435 WP_002887280.1 NZ_CP043344:c4731972-4731439 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T138435 NZ_CP057864:c1188377-1187997 [Citrobacter freundii]
ATGCAAACTTTATCGTTATCCGAGCCACGGGCGGCTGAGCCATGCCCGTCGCCCGTGCAGGTCTGGCAGCTGCTGCTCAT
ACATCTGCTAGATAAACATTATGGCCTCACGCTCAACGATACGCCGTTTGGCGATGACAGCGTAATTCAGGCGCACATCA
ACGCCCGTATCTCTCTGTGTGATGCTGTCAATTTCATTGTGCAAAGGTATGAACTGGTGCGCACCGACAACAGCAGTTTT
AACCTTAGCGATCGCTCATCGCTAATTGGTGCCATCGACATTCTGCATGCCCGTCGTGCAACCGGACTGATAGTAAGAAG
CGGCTATAGCAACATAACCCGCATCACAACGGGCCGGTATCAAGGAGGAGAAGCACAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT138435 WP_002887278.1 NZ_CP043344:c4732249-4731983 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT138435 NZ_CP043344:c4732249-4731983 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References