Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4118749..4119368 Replicon chromosome
Accession NZ_CP043344
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CVC44_RS20325 Protein ID WP_002892050.1
Coordinates 4119150..4119368 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CVC44_RS20320 Protein ID WP_002892066.1
Coordinates 4118749..4119123 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVC44_RS20310 (CVC44_20490) 4113901..4115094 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CVC44_RS20315 (CVC44_20495) 4115117..4118263 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
CVC44_RS20320 (CVC44_20500) 4118749..4119123 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CVC44_RS20325 (CVC44_20505) 4119150..4119368 + 219 WP_002892050.1 HHA domain-containing protein Toxin
CVC44_RS20330 (CVC44_20510) 4119527..4120093 + 567 WP_002892030.1 maltose O-acetyltransferase -
CVC44_RS20335 4120065..4120205 - 141 WP_004147370.1 hypothetical protein -
CVC44_RS20340 (CVC44_20515) 4120226..4120696 + 471 WP_002892026.1 YlaC family protein -
CVC44_RS20345 (CVC44_20520) 4120671..4122122 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CVC44_RS20350 (CVC44_20525) 4122223..4122921 + 699 WP_002892021.1 GNAT family protein -
CVC44_RS20355 (CVC44_20530) 4122918..4123058 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CVC44_RS20360 (CVC44_20535) 4123058..4123321 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138434 WP_002892050.1 NZ_CP043344:4119150-4119368 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T138434 NZ_CP043344:4119150-4119368 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT138434 WP_002892066.1 NZ_CP043344:4118749-4119123 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT138434 NZ_CP057864:826867-827133 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGAGGTATGCGCGAGCTGGATATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References