Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 2348..2949 Replicon plasmid p2_115106
Accession NZ_CP043337
Organism Escherichia coli strain WCHEC115106

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag FZC46_RS24455 Protein ID WP_001216045.1
Coordinates 2569..2949 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag FZC46_RS24450 Protein ID WP_001190712.1
Coordinates 2348..2569 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZC46_RS24430 (FZC46_24590) 513..752 + 240 WP_023356286.1 DNA polymerase III subunit theta -
FZC46_RS24435 (FZC46_24595) 764..1126 - 363 WP_042102765.1 hypothetical protein -
FZC46_RS24440 (FZC46_24600) 1127..1711 - 585 WP_023356284.1 hypothetical protein -
FZC46_RS24445 (FZC46_24605) 1886..2275 + 390 WP_000506730.1 S24 family peptidase -
FZC46_RS24450 (FZC46_24610) 2348..2569 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FZC46_RS24455 (FZC46_24615) 2569..2949 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FZC46_RS24460 (FZC46_24620) 2954..3133 + 180 WP_001339207.1 hypothetical protein -
FZC46_RS24465 (FZC46_24625) 3161..4204 + 1044 WP_023352824.1 DUF968 domain-containing protein -
FZC46_RS24470 (FZC46_24630) 4293..4745 + 453 WP_001326849.1 late promoter-activating protein -
FZC46_RS24475 (FZC46_24635) 4831..6024 + 1194 WP_029487616.1 hypothetical protein -
FZC46_RS24480 (FZC46_24640) 6024..7508 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..90994 90994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T138402 WP_001216045.1 NZ_CP043337:2569-2949 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T138402 NZ_CP043337:2569-2949 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT138402 WP_001190712.1 NZ_CP043337:2348-2569 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT138402 NZ_CP043337:2348-2569 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References