Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 125081..125724 Replicon plasmid p1_115106
Accession NZ_CP043336
Organism Escherichia coli strain WCHEC115106

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag FZC46_RS24390 Protein ID WP_001044768.1
Coordinates 125308..125724 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag FZC46_RS24385 Protein ID WP_001261287.1
Coordinates 125081..125311 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZC46_RS24360 (120995) 120995..121183 + 189 WP_048230572.1 hypothetical protein -
FZC46_RS24365 (121193) 121193..122392 + 1200 WP_000948429.1 IS91 family transposase -
FZC46_RS24370 (122548) 122548..123294 - 747 WP_241420249.1 hypothetical protein -
FZC46_RS24375 (123345) 123345..124175 - 831 WP_223201786.1 hypothetical protein -
FZC46_RS24380 (124227) 124227..124775 - 549 WP_039002808.1 hypothetical protein -
FZC46_RS24385 (125081) 125081..125311 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FZC46_RS24390 (125308) 125308..125724 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
FZC46_RS24395 (125886) 125886..128024 - 2139 WP_039002805.1 AAA family ATPase -
FZC46_RS24400 (128489) 128489..129484 + 996 WP_000246636.1 hypothetical protein -
FZC46_RS24405 (129488) 129488..130420 + 933 WP_000991832.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / aac(3)-IId - 1..133446 133446
- flank IS/Tn - - 130557..131060 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T138401 WP_001044768.1 NZ_CP043336:125308-125724 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T138401 NZ_CP057682:74015-74167 [Escherichia fergusonii]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT138401 WP_001261287.1 NZ_CP043336:125081-125311 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT138401 NZ_CP057682:c73965-73903 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References