Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 53017..53552 Replicon plasmid p1_115106
Accession NZ_CP043336
Organism Escherichia coli strain WCHEC115106

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag FZC46_RS23930 Protein ID WP_000222766.1
Coordinates 53017..53304 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag FZC46_RS23935 Protein ID WP_001132900.1
Coordinates 53301..53552 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZC46_RS23900 (48565) 48565..49425 - 861 WP_000557454.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
FZC46_RS23905 (49568) 49568..50062 + 495 Protein_57 IS4-like element ISVsa5 family transposase -
FZC46_RS23910 (50116) 50116..50820 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FZC46_RS23915 (50888) 50888..52186 + 1299 Protein_59 Tn3-like element Tn3 family transposase -
FZC46_RS23920 (52220) 52220..52720 + 501 Protein_60 Tn3 family transposase -
FZC46_RS23925 (52754) 52754..52942 + 189 Protein_61 IS3 family transposase -
FZC46_RS23930 (53017) 53017..53304 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FZC46_RS23935 (53301) 53301..53552 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FZC46_RS23940 (54518) 54518..55375 - 858 WP_039002220.1 incFII family plasmid replication initiator RepA -
FZC46_RS23945 (55368) 55368..55442 - 75 WP_061357229.1 RepA leader peptide Tap -
FZC46_RS23950 (55674) 55674..55931 - 258 WP_001541895.1 replication regulatory protein RepA -
FZC46_RS23955 (56199) 56199..56348 - 150 WP_001312851.1 Hok/Gef family protein -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
FZC46_RS23960 (56795) 56795..57007 - 213 WP_001541896.1 ANR family transcriptional regulator -
FZC46_RS23965 (57142) 57142..57702 - 561 WP_000139310.1 fertility inhibition protein FinO -
FZC46_RS23970 (57757) 57757..58209 - 453 WP_241420250.1 type-F conjugative transfer system pilin acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / aac(3)-IId - 1..133446 133446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T138393 WP_000222766.1 NZ_CP043336:c53304-53017 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T138393 NZ_CP057681:c2564653-2564501 [Escherichia fergusonii]
ATGCCGCAGAAATATAGATTACTTTCTTTAATAGTGATTTGTTTCACACTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAATTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT138393 WP_001132900.1 NZ_CP043336:c53552-53301 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT138393 NZ_CP043336:c53552-53301 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References