Detailed information of TA system TA138378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1344443..1345068
• Replicon: chromosome
• Accession: NZ_CP043334
• Organism: Escherichia coli strain WCHEC115106

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FZC46_RS06530
• Protein ID: WP_000911330.1
• Coordinates: 1344670..1345068 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FZC46_RS06525
• Protein ID: WP_000450524.1
• Coordinates: 1344443..1344670 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZC46_RS06500 (1340246)	1340246..1340716	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FZC46_RS06505 (1340716)	1340716..1341288	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FZC46_RS06510 (1341434)	1341434..1342312	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FZC46_RS06515 (1342329)	1342329..1343363	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FZC46_RS06520 (1343576)	1343576..1344289	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FZC46_RS06525 (1344443)	1344443..1344670	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FZC46_RS06530 (1344670)	1344670..1345068	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FZC46_RS06535 (1345215)	1345215..1346078	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
FZC46_RS06540 (1346093)	1346093..1348108	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FZC46_RS06545 (1348182)	1348182..1348523	+	342	Protein_1284	esterase	-
FZC46_RS06550 (1348605)	1348605..1349351	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T138378 WP_000911330.1 NZ_CP043334:1344670-1345068 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T138378 NZ_CP043334:1344670-1345068 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT138378 WP_000450524.1 NZ_CP043334:1344443-1344670 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT138378 NZ_CP043334:1344443-1344670 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33