Detailed information of TA system TA138368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3322113..3323134
• Replicon: chromosome
• Accession: NZ_CP043332
• Organism: Proteus mirabilis strain CRPM10

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: FY167_RS15535
• Protein ID: WP_149127700.1
• Coordinates: 3322565..3323134 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: FY167_RS15530
• Protein ID: WP_004245528.1
• Coordinates: 3322113..3322565 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY167_RS15510	3317899..3318429	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
FY167_RS15515	3318698..3319711	+	1014	WP_041701263.1	thiamine ABC transporter substrate binding subunit	-
FY167_RS15520	3319711..3321291	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
FY167_RS15525	3321284..3321985	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
FY167_RS15530	3322113..3322565	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
FY167_RS15535	3322565..3323134	+	570	WP_149127700.1	PIN domain-containing protein	Toxin
FY167_RS15540	3323250..3325604	+	2355	WP_149127701.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21673.15 Da
• Isoelectric Point: 6.7372

>T138368 WP_149127700.1 NZ_CP043332:3322565-3323134 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKEQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T138368 NZ_CP057657:223078-223181 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT138368 WP_004245528.1 NZ_CP043332:3322113-3322565 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT138368 NZ_CP043332:3322113-3322565 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3