Detailed information of TA system TA138362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4745745..4747003
• Replicon: chromosome
• Accession: NZ_CP043328
• Organism: Pseudomonas aeruginosa strain CCUG 51971

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: FZE14_RS22915
• Protein ID: WP_003086331.1
• Coordinates: 4745745..4746500 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: FZE14_RS22920
• Protein ID: WP_003086329.1
• Coordinates: 4746635..4747003 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FZE14_RS22905	4740916..4743459	+	2544	WP_003134033.1	acyl-homoserine-lactone acylase QuiP	-
FZE14_RS22910	4743614..4744975	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
FZE14_RS22915	4745745..4746500	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
FZE14_RS22920	4746635..4747003	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
FZE14_RS22925	4747076..4748362	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
FZE14_RS22930	4748418..4750007	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
FZE14_RS22935	4750086..4750562	+	477	WP_003086322.1	hypothetical protein	-
FZE14_RS34080	4750565..4750840	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T138362 WP_003086331.1 NZ_CP043328:c4746500-4745745 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T138362 NZ_CP057632:c2837080-2836977 [Citrobacter sp. RHB20-C15]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT138362 WP_003086329.1 NZ_CP043328:c4747003-4746635 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT138362 NZ_CP057632:2836973-2837120 [Citrobacter sp. RHB20-C15]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCTGCG

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7