Detailed information of TA system TA138324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 53153..53741
• Replicon: plasmid pAcsw19-2
• Accession: NZ_CP043309
• Organism: Acinetobacter johnsonii strain Acsw19

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: FYN22_RS16810
• Protein ID: WP_000438825.1
• Coordinates: 53454..53741 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: FYN22_RS16805
• Protein ID: WP_004728120.1
• Coordinates: 53153..53467 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYN22_RS16770	48609..50084	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FYN22_RS16775	50577..50849	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FYN22_RS16780	50842..51162	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYN22_RS16785	51302..51721	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
FYN22_RS16790	51801..52082	-	282	WP_000985609.1	putative addiction module antidote protein	-
FYN22_RS16795	52083..52385	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYN22_RS16800	52534..53064	+	531	WP_000172759.1	hypothetical protein	-
FYN22_RS16805	53153..53467	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
FYN22_RS16810	53454..53741	-	288	WP_000438825.1	BrnT family toxin	Toxin
FYN22_RS16815	53971..54576	-	606	WP_000447788.1	LysE family translocator	-
FYN22_RS16825	55815..56642	-	828	WP_000073658.1	AraC family transcriptional regulator	-
FYN22_RS16830	56770..57522	-	753	Protein_62	IS1-like element ISAba3 family transposase	-
FYN22_RS16835	57613..58455	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlB1 / mph(E) / msr(E) / blaOXA-58 / blaNDM-1 / aph(3')-VIa	-	1..351885	351885
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	45650..62451	16801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T138324 WP_000438825.1 NZ_CP043309:c53741-53454 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T138324 NZ_CP057565:c3550638-3550231 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCAGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT138324 WP_004728120.1 NZ_CP043309:c53467-53153 [Acinetobacter johnsonii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT138324 NZ_CP057565:c3550885-3550619 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAAATGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9