138324

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 53153..53741 Replicon plasmid pAcsw19-2
Accession NZ_CP043309
Organism Acinetobacter johnsonii strain Acsw19

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag FYN22_RS16810 Protein ID WP_000438825.1
Coordinates 53454..53741 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag FYN22_RS16805 Protein ID WP_004728120.1
Coordinates 53153..53467 (-) Length 105 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYN22_RS16770 48609..50084 - 1476 WP_000052512.1 ABC-F type ribosomal protection protein Msr(E) -
FYN22_RS16775 50577..50849 - 273 WP_000369781.1 helix-turn-helix domain-containing protein -
FYN22_RS16780 50842..51162 - 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
FYN22_RS16785 51302..51721 + 420 WP_001180106.1 SMI1/KNR4 family protein -
FYN22_RS16790 51801..52082 - 282 WP_000985609.1 putative addiction module antidote protein -
FYN22_RS16795 52083..52385 - 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
FYN22_RS16800 52534..53064 + 531 WP_000172759.1 hypothetical protein -
FYN22_RS16805 53153..53467 - 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
FYN22_RS16810 53454..53741 - 288 WP_000438825.1 BrnT family toxin Toxin
FYN22_RS16815 53971..54576 - 606 WP_000447788.1 LysE family translocator -
FYN22_RS16825 55815..56642 - 828 WP_000073658.1 AraC family transcriptional regulator -
FYN22_RS16830 56770..57522 - 753 Protein_62 IS1-like element ISAba3 family transposase -
FYN22_RS16835 57613..58455 - 843 WP_002002480.1 carbapenem-hydrolyzing class D beta-lactamase OXA-58 -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cmlB1 / mph(E) / msr(E) / blaOXA-58 / blaNDM-1 / aph(3')-VIa - 1..351885 351885
- inside IScluster/Tn mph(E) / msr(E) / blaOXA-58 - 45650..62451 16801


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T138324 WP_000438825.1 NZ_CP043309:c53741-53454 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T138324 NZ_CP057565:c3550638-3550231 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCAGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT138324 WP_004728120.1 NZ_CP043309:c53467-53153 [Acinetobacter johnsonii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT138324 NZ_CP057565:c3550885-3550619 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAAATGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4


Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References