Detailed information of TA system TA138323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 51801..52385
• Replicon: plasmid pAcsw19-2
• Accession: NZ_CP043309
• Organism: Acinetobacter johnsonii strain Acsw19

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: FYN22_RS16795
• Protein ID: WP_000286964.1
• Coordinates: 52083..52385 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FYN22_RS16790
• Protein ID: WP_000985609.1
• Coordinates: 51801..52082 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYN22_RS16760	46868..47479	+	612	WP_015060246.1	recombinase family protein	-
FYN22_RS16765	47669..48553	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FYN22_RS16770	48609..50084	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FYN22_RS16775	50577..50849	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FYN22_RS16780	50842..51162	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYN22_RS16785	51302..51721	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
FYN22_RS16790	51801..52082	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
FYN22_RS16795	52083..52385	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYN22_RS16800	52534..53064	+	531	WP_000172759.1	hypothetical protein	-
FYN22_RS16805	53153..53467	-	315	WP_004728120.1	BrnA antitoxin family protein	-
FYN22_RS16810	53454..53741	-	288	WP_000438825.1	BrnT family toxin	-
FYN22_RS16815	53971..54576	-	606	WP_000447788.1	LysE family translocator	-
FYN22_RS16825	55815..56642	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	cmlB1 / mph(E) / msr(E) / blaOXA-58 / blaNDM-1 / aph(3')-VIa	-	1..351885	351885
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	45650..62451	16801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T138323 WP_000286964.1 NZ_CP043309:c52385-52083 [Acinetobacter johnsonii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T138323 NZ_CP057565:c3466807-3466409 [Escherichia fergusonii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGACAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTTGCGATGCACTCTATGACGACTTGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAAAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT138323 WP_000985609.1 NZ_CP043309:c52082-51801 [Acinetobacter johnsonii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT138323 NZ_CP057565:c3467103-3466810 [Escherichia fergusonii]
ATGCATCGAATTCTTGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGTGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94