Detailed information of TA system TA138297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 53135..53760
• Replicon: plasmid p1_040047
• Accession: NZ_CP043297
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FYK54_RS25140
• Protein ID: WP_000911313.1
• Coordinates: 53362..53760 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: FYK54_RS25135
• Protein ID: WP_000450520.1
• Coordinates: 53135..53362 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS25135 (53135)	53135..53362	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FYK54_RS25140 (53362)	53362..53760	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
FYK54_RS25145 (53769)	53769..54125	-	357	Protein_66	conjugal transfer protein TraD	-
FYK54_RS25150 (54162)	54162..54839	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
FYK54_RS25155 (54839)	54839..55186	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FYK54_RS25160 (55206)	55206..56777	+	1572	WP_029392005.1	IS66-like element ISCro1 family transposase	-
FYK54_RS25165 (56809)	56809..58629	-	1821	Protein_70	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..110724	110724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T138297 WP_000911313.1 NZ_CP043297:53362-53760 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T138297 NZ_CP057509:4288446-4288733 [Citrobacter freundii]
ATGAGTTATACGGTAAAGTTCAGGGAAGATGCGCTGAAAGAATGGCAAAAATTGGATAAATCCATTCAGCAGCAGTTTGC
CAAAGAACTTAAGAAATGCGGTGAAAATCCGCATGTCCCCTCCGCGAAGCTCAGGGGAATAAAAGATTGCTATATAATCC
AACTACGCACTTCAAGGTTCCGTCTTGTTTATCAAGTTATTGACGACACCCTGGTCATCGCCGTCGTTGCTGTCGGTAAA
CGCGAGCGTAGTGAAGTTTATAATCTTGCCAGCGAAAGACTACGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT138297 WP_000450520.1 NZ_CP043297:53135-53362 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT138297 NZ_CP057509:4288198-4288449 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCGAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAGCCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2