Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21104..21747 Replicon plasmid p1_040047
Accession NZ_CP043297
Organism Escherichia coli strain WCHEC040047

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag FYK54_RS24940 Protein ID WP_001044768.1
Coordinates 21331..21747 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag FYK54_RS24935 Protein ID WP_001261287.1
Coordinates 21104..21334 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK54_RS24915 (16264) 16264..17352 + 1089 WP_000952217.1 transcriptional repressor PifC -
FYK54_RS24920 (17354) 17354..19579 + 2226 WP_000698737.1 P-loop NTPase fold protein -
FYK54_RS24925 (19629) 19629..20528 - 900 WP_000963206.1 nucleotide-binding protein -
FYK54_RS24930 (20518) 20518..20808 - 291 WP_000111771.1 hypothetical protein -
FYK54_RS24935 (21104) 21104..21334 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FYK54_RS24940 (21331) 21331..21747 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
FYK54_RS24945 (21909) 21909..23297 - 1389 Protein_26 AAA family ATPase -
FYK54_RS24950 (23361) 23361..24065 + 705 WP_001067834.1 IS6-like element IS26 family transposase -
FYK54_RS24955 (24437) 24437..24886 - 450 WP_039021304.1 hypothetical protein -
FYK54_RS24960 (24897) 24897..25436 - 540 WP_039021305.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIa / sitABCD iutA / iucD / iucC / iucB / iucA 1..110724 110724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T138292 WP_001044768.1 NZ_CP043297:21331-21747 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T138292 NZ_CP057509:c1209937-1209596 [Citrobacter freundii]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGACACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAACACTATGGTCTTACCCTGAACGATACTCCTTTTATTGATGAAACCGTTATTCAGAATCACATTA
ATGCCGGGATCACGCTTGCTGATGCCGTTAACTTTCTGGTGGATAAGTACGAGCTTGTTCGCATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAA
CCGTAACAATGCAGTACGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT138292 WP_001261287.1 NZ_CP043297:21104-21334 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT138292 NZ_CP043297:21104-21334 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References